|
Descrição |
|
|
-
ANA CRISTINA CARDOSO SANTOS
-
Análise in vitro das propriedades citotóxicas do extrato de Andiroba (Carapa guianensis) frente ao Herpes Vírus Simples- tipo 1 (HSV-1)
-
Data: 08/11/2023
-
Mostrar Resumo
-
Herpes Simplex is an infection caused by the human herpes virus (HSV), and is one of the most common infections of humans. Contaminated saliva constitutes an efficient vehicle for the transmission of HSV-1, in addition to possible contact with herpetic secretions in people who clinically present the disease. Carapa guianensis, popularly known as Andiroba, is a large tree of the Meliaceae family, found in the Amazon region. Andiroba oil is extracted by pressing its seeds and has the main biologically active substances and provides numerous effects, especially anti-inflammatory effects. This set of characteristics has attracted the attention of some researchers to study the action of Andiroba in various treatments, however so far there is no research that seeks as an alternative option for oral lesions by HSV-1 and the use of herbal medicines, and no study showed the effect and effectiveness of the use of andiroba oil in these lesions, therefore, this work would be the pioneer to evaluate these antiviral characteristics and cell viability. The objective of this study is to evaluate the acute toxicity (LC50) and behavioral effects of Andiroba (Carapa Guianeses) against Herpes Simplex Virus-type 1. This is an experimental laboratory study, using HSV-1 viral strains. To evaluate the effective cytotoxicity of Andiroba oil, we will use VERO E6 cells, permissible for HSV-1. To test cell viability, the Trypan blue dye exclusion method and the MTT colorimetric assay (3-(4,5-dimethyl-2-thiazole)-2,5-diphenyl-2-H-tetrazolium bromide) will be used. Andiroba oil did not cause significant damage to the DNA of VERO E6 cells, at the concentrations tested (10µg/ml and 100µg/ml), when compared to the positive control (Tukey test p>0.05); The concentrations (10µg/ml and 100µg/ml) did not damage cellular DNA and are statistically similar to each other (Tukey test p>0.05). The data analyzed showed that Andiroba oil, in both concentrations, did not interfere with the viability of the cells in question, so there was no difference between the treatments tested, and therefore, no toxicity was demonstrated in the treatments performed.
Keywords: Andiroba: Carapa guianensis; Herpes Simplex; Cytotoxicity.
|
|
|
-
ANA CRISTINA ALVES DA SILVA
-
PERFIL EPIDEMIOLÓGICO DE MIELOMA MÚLTIPLO A LONGO PRAZO EM PACIENTES ATENDIDOS EM HOSPITAL ONCOLÓGICO DE BELÉM-PA
-
Data: 06/11/2023
-
Mostrar Resumo
-
The objective of this study was to analyze epidemiological, clinical and laboratory data from patients diagnosed with multiple myeloma (MM) treated at Ophir Loyola Hospital. This is a descriptive epidemiological study, carried out on medical records of patients diagnosed with multiple myeloma, at the Ophir Loyola Hospital in the city of Belém-PA, between 2002 and 2020. Sociodemographic variables, clinical characteristics, laboratory and imaging tests, location and progression of the injury, treatment and follow-up in the Oral and Maxillofacial Surgery and Traumatology sector were considered. Descriptive statistics were used to describe the absolute and relative frequency of groups or categories for each observed variable. To evaluate the association between the main variables and other variables in their different groups or categories, Pearson's Chi-square and/or Fisher's Exact tests were used to compare the proportions of responses by groups or categories. Of the 206 patients evaluated, 48.5% were male and 51.5% female, 50% of patients were aged between 41 and 60 years and 43.7% were mixed race, 28.2% of patients were former consumers of alcohol and 37.9% former tobacco consumers. In relation to the international system, 47 (22.8%) patients were in ISS III, 22 (10.7%) in ISS II and 19 (9.2%) in ISS I and 118 (57.3% ) uninformed. The international staging system (ISS) did not show statistical relevance in relation to the variables evaluated. When evaluating addictions, patients who were former alcohol and tobacco consumers showed a higher percentage of IgG protein compared to patients who did not have addictions. When evaluating the history of malignancy in the family, a higher prevalence of kappa light chain was observed compared to lambda. IgA has a higher percentage in the lambda chain and IgG has a higher percentage in the kappa chain. This study made it possible to establish an epidemiological profile of patients diagnosed with MM in a reference hospital for oncology in the state of Pará, determining prognostic factors that directly contribute to greater knowledge of the disease on the part of health professionals, early diagnosis and management of the disease .
Keywords: Monoclonal gammopathies. Myelogram. Monoclonal protein. Oral tumors.
|
|
|
-
MARA ELIANE SOARES RIBEIRO
-
TRATAMENTOS PÓS-CLAREAMENTO DENTAL COM GÉIS DE ALTA CONCENTRAÇÃO CONTENDO CÁLCIO OU FLUORETO DE SÓDIO AFETAM A SUPERFÍCIE DO ESMALTE DENTAL?
-
Data: 23/10/2023
-
Mostrar Resumo
-
Objective: To evaluate the influence of dental polishing and topical application of neutral fluoride after bleaching performed with 35% hydrogen peroxide containing calcium (PH35), or with 40% hydrogen peroxide containing fluoride (PH40), on surface loss and enamel ultrastructure.
Methodology: 108 healthy bovine incisor teeth were divided into 9 groups (n=12): negative control with artificial saliva (CN); Tooth bleaching with PH35% (PH35); Tooth bleaching with PH40% (PH40); Tooth bleaching with PH35%+ dental polishing with diamond paste (PH35Po); Tooth bleaching with PH40%+ dental polishing with diamond paste (PH40Po); Tooth bleaching with PH35% + topical application of fluoride (PH35F); Tooth bleaching with PH40% + topical application of fluoride (PH40F); Tooth bleaching PH35%+ dental polishing with diamond paste+ topical application of fluoride (PH35PoF); Tooth bleaching PH40%+ dental polishing with diamond paste+ topical application of fluoride (PH40PoF). For the analysis of structure loss, the specimens were read before (baseline) and after the treatments in each group. For the ultrastructure, two specimens from each group were selected, from which images were obtained by scanning electron microscopy (SEM). The surface loss data showed normal distribution, and were submitted to the two-way anova test with Tukey's post-test. The ultrastructure was analyzed descriptively from the images obtained.
Results: Statistical comparisons were performed independently for groups of different bleaching gels. Comparisons between groups for PH40 showed statistically significant difference (p˂0.01) between CN and PH40Po, PH40 and PH40Po, PH40Po both with PH40F and PH40PoF. Comparisons for groups with PH35, we observed the differences for my treatments- CN and PH35Po, PH35 and PH35Po, PH35Po both with PH35F and PH35PoF.
Conclusion: tooth bleaching, regardless of the concentration used, caused less surface loss, and fluoride as a post-treatment seems to have a protective effect when dental polishing is associated with the technique.
Keywords: tooth bleaching, tooth enamel, hydrogen peroxide, sodium fluoride, SEM.
|
|
|
-
ALINE MARQUES DIAS
-
ANÁLISE DA EXPRESSÃO DE METALOPROTEINASE-2, -9, METALOTIONEÍNA, FATOR DE CRESCIMENTO ENDOTELIAL VASCULAR E SEU RECEPTOR EM ADENOMA PLEOMÓRFICO
-
Data: 26/09/2023
-
Mostrar Resumo
-
Pleomorphic adenoma (PA) is the most common neoplasm of the salivary glands. The main characteristics of PA are the high frequency of occurrence and local invasiveness. Metalloproteinases -2 (MMP-2), -9 (MMP-9) and metallothionein (MT) are strongly associated with the local invasiveness of several tumors, but there are no reports on this correlation in PA. It is suggested that PA cells produce vascular endothelial growth factor (VEGF), which exerts its function linked to its receptor (VEGFR) and has a main role in tumor angiogenesis, cell proliferation and migration, events that are also important for tumor invasiveness. Therefore, the aim of this study was to investigate the expression of MMP-2, MMP-9, MT, VEGF and VEGFR, and to verify the possible correlation between their expression and the local invasiveness of PA. This study, unprecedented in the literature, is extremely important for a better understanding of the biological behavior of this neoplasm. Cell culture of normal PA and salivary gland (SG) cell lines was carried out to conduct the transcriptomic analysis, in addition to observing the protein-protein interaction network with the Search Tool for the Retrieval of Interacting Genes/Proteins (STRING). The immunoexpression of the proteins of interest in the study was also observed in PA and SG, with subsequent evaluation of the immunostaining with the ImageJ software. In addition to descriptive statistics, the GraphPadPrism 5 software was used to analyze the data obtained, with Student's T and Mann-Whitney tests being performed, in addition to Pearson and Spearman correlations. In the transcriptomic analysis, it was observed that MT2A, MMP-2, VEGF and VEGFR presented a higher number of reads in PA when compared to SG. In the protein-protein interaction network, a direct link between MT2A and MMPs was observed, with MMPs providing an indirect interaction between MT2A and angiogenic factors. It was verified that PA presented high immunoexpression of MMP-2, MMP-9, MT, VEGF and VEGFR when compared to SG. There was a positive correlation between MMP-9 and MT and VEGF. It is suggested that MMP-9 actively participates in the mechanisms of PA invasiveness, in association with the other proteins studied.
Keywords: Adenoma, Pleomorphic; Salivary Gland Neoplasms; Metallothionein; Matrix Metalloproteinases; Vascular Endothelial Growth Factor.
|
|
|
-
NAYARA CRISTINA MONTEIRO CARNEIRO
-
"Acurácia cirúrgica de um novo modelo de guia maxilar osseo-dento-suportado em cirurgia ortognática: método de benefício antecipado"
-
Data: 04/09/2023
-
Mostrar Resumo
-
This research aims to evaluate and validate the surgical accuracy of a new type of bone-tooth-supported maxillary splint (SMOB) made using CAD/CAM technology, which has a dual function, that of a maxillary positioning guide and that of a cutting guide to Le Fort I osteotomy in combined orthognathic surgeries for “surgery first approach”. This is a prospective study carried out in patients with dentofacial skeletal deformities at Hospital Ophir Loyola/PA, who underwent surgical correction between the years 2018 to 2022 and selected within the eligibility criteria. For all cases included in the study, the planning and virtual surgery software used was Dolphin Imaging Solutions® (California, United States). The images of the planning skulls were transferred to the Blender® 2.80 software (Amsterdam, Netherlands) for the creation of the customized guide in an STL file (Standard Triangle Language) and subsequent three-dimensional (3D) printing. The accuracy of the SMOB guide was made based on the superposition of the maxillary planning image (CT0) to the postoperative 3D tomographic reconstruction (CT1). The average differences between them were quantified in the mediolateral (X), anteroposterior (Y) and vertical (Z) movements, in six different maxillary points, being three bony and three dental, so that a more careful evaluation of the accuracy could be performed in a three-dimensional way. To compare the planning (CT0) and postoperative (CT1) images, the T-Student test was chosen at each of the six different points and movements performed. For abnormal samples, the wilcoxon test was chosen. Another analysis used in this research was the Bland and Altman method, with a 95% confidence interval.The SODM guides results suggests a better accuracy Os resultados do guia SODM sugerem que o mesmo apresentou uma boa acurácia nos três planos maxilares, X, Y e Z, com discrepâncias abaixo de 1 mm em todos os pontos avaliados However, the Y axis showed the highest standard deviations among the samples, with the guide having the lowest precision in this movement. On the Z axis, only one of the dental points showed a statistically significant difference between CT0 and CT1. The best accuracy results were observed in the mediolateral plane. More studies comparing other methods have to be performed so that the SODM guide has its accuracy and effectiveness validated.
Keywords: orthognathic surgery; three-dimensional printing; jaw; osteotomy.
|
|
|
-
DOUGLAS VOSS DE OLIVEIRA
-
Guia maxilar CAD/CAM para osteotomia, perfuração e posicionamento maxilar em cirurgia ortognática: análise de acurácia
-
Data: 04/09/2023
-
Mostrar Resumo
-
Resin CAD/CAM guides for the maxilla are widely used and differ from custom miniplates essentially in that they do not have drill guides for screw fixation and use miniplates that must be modeled in surgery. To solve this problem, we have developed a new Maxillary guide bone-supported indicated for osteotomy, drilling, and maxillary positioning in orthognathic surgeries using standard modeled miniplates. In this study were included sixteen patients who had bimaxillary orthognathic surgery. The technique for its manufacturing is described, and its surgical accuracy was analyzed using the planning image (CT0) superimposed on the computed tomography scan post-treatment (CT1). The mean positional differences between pre-treatment and post-treatment were obtained Tri-dimensionally on the X, Y, and Z axes using four landmarks points: one bone (SNA), and three dentals (CI, RM, and LM). At Sixty-four points analyzed on the X axis of the four landmarks, only eight points were greater than 1 mm; 87.5 % of the deviations were <1 mm. For the Y-axis 78.12% of the deviations were <1 mm and for Z-axis, 76.56 %. The mean and standard deviation of the error was 0.36 (0.28) mm, 0.93 (1.07) mm, and 0.73 (0.86) mm on the X, Y, and Z axes, respectively. The X-axis demonstrated the best results among the three axes. The results of the accuracy and reliability tests were satisfactory and support the use of the proposed technique.
Keywords: Orthognathic Surgery; Osteotomy; Le Fort; Splints; Surgical Fixation Devices; Computer-Aided Design
|
|
|
-
RENATA GUIMARÃES GONÇALVES ROCHA
-
AVALIAÇÃO DA CITOTOXICIDADE, ATIVIDADE ANTIMICROBIANA DO ÓLEO DE PRACAXI (Pentaclethra macroloba Kuntze) IN VITRO E EFEITO CICATRIZANTE DA EMULSÃO À BASE DE PRACAXI NA CICATRIZAÇÃO DE FERIDAS PALATINAS IN VIVO
-
Data: 17/07/2023
-
Mostrar Resumo
-
Objetivo: O estudo avaliou a atividade citotóxica e antibacteriana in vitro do óleo de pracaxi (Pentaclethra macroloba Kuntze), assim como em emulsão na cicatrização em modelos de ferida palatina de ratos Wistar.
Material e Métodos: O óleo de pracaxi foi caracterizado por espectrometria e os ensaios de citotoxicidade em fibroblastos humanos imortalizados e atividade antibacteriana foram analisados, respectivamente, por ensaio de MTT e em disco-difusão contra as bactérias Staphylococcus aureus, Escherichia coli e Klebsiella pneumoniae. Para a avaliação do efeito cicatricial da emulsão de quitosana e pracaxi a 3%, 48 animais foram alocados aleatoriamente em 3 grupos (Controle, Quitosana e Pracaxi) após a confecção de ferida palatina de 3 mm de diâmetro. As aplicações tópicas dos tratamentos foram realizadas imediatamente após a cirurgia e a cada 2 dias durante 7 dias. A avaliação da cicatrização foi realizada por macroscopia, histomorfometria e avaliação histopatológica (escores de re-epitelização, tecido de granulação e formação de fibras colágenas).
Resultados: O óleo de pracaxi não apresentou citotoxicidade aos fibroblastos humanos, nem atividade antimicrobiana para S. aureus, E. coli. ou K. pneumoniae. A área da ferida macroscópica evidenciou diminuição significativa (p<0.001-ANOVA One-way). Na histomorfometria, também houve diferença significativa (p<0.001) quanto ao fechamento da ferida para o grupo tratado com emulsão de pracaxi. Com relação à análise histopatológica por scores, não foram observadas diferenças estatísticas significativas no período observado, apesar da maior homogeneidade de resposta no grupo exposto à emulsão de pracaxi em relação ao grupo controle.
Conclusões: O óleo de pracaxi testado não interferiu na viabilidade das células humanas e não apresentou sensibilidade às bactérias testadas. Foi observada que a emulsão de quitosana à base de pracaxi promoveu uma redução significativa da área da ferida e da distância dos bordos epiteliais, mas não foram observadas diferença significativa nas análises histopatológicas de reepitelização, formação de tecido de granulação e formação de nova fibras colágenas.
|
|
|
-
ANA PAULA GUERREIRO MATTOS RODRIGUES
-
COMPARAÇÃO DOS PADRÕES DAS FRATURAS EM OSTEOTOMIAS DO TIPO SAGITAL CONVENCIONAL E MODIFICADA POR WOLFORD: Ensaio Laboratorial de Tração em Mandíbulas de Cadáveres.
-
Orientador : SERGIO DE MELO ALVES JUNIOR
-
Data: 30/06/2023
-
Mostrar Resumo
-
The sagittal osteotomy of the mandibular ramus (OSRM) is the most commonly performed osteotomy for the resolution of complex cases of dentofacial deformities, during orthognathic surgery, because it allows for surgical movements of mandibular advancement and retreat. This procedure, of high technical expertise, was popularized by Trauner and Obwegeser in 1957, and modified until the present day, always with the objective of minimizing operative intercurrences. The aim of the present study was to compare the fracture patterns, and the torque required for the mandibular sagittal split according to the Epker and modified Wolford techniques. The methodology of this study consisted of a randomized survey, osteotomies on both sides of 61 mandibles of human cadavers, which were divided in their midline, totaling 122 osteotomies procedures (hemimandibles), separated into 2 groups: Group 1 - 61 osteotomies conventional sagittal type (Epker); Group 2 - 61 osteotomies of the sagittal type modified by Wolford. They were submitted to a traction test on the KRATO Universal Testing Machine to measure the traction force during their separation, and the lingual fracture pattern of each division was classified. All results were examined and the level of significance (p ) was set at p<0.05. The tensile strength forces were significantly lower (p<0.001) when using the Wolford technique when compared to the Epker technique (conventional). The Conventional group (Epker) had a higher prevalence of type IV fracture, which is considered an unwanted fracture (p<0.001). While in the modified Wolford group, a higher frequency of type I fractures was found, which would be the most desired fracture for this type of surgical procedure. There was also no significant correlation between tensile strength and jaw thickness. In conclusion, sagittal osteotomy using the modified Wolford technique reduces the possibility of unwanted fracture of the mandible and damage to the inferior alveolar nerve, in addition to producing more predictable results with reduced resistance force.
KEY WORDS: Bad split. Mandibular osteotomy. Maxillofacial surgery. Orthognathic surgery. Osteotomy
|
|
|
-
ISSAE SOUSA SANO
-
"EFFECT OF AN EXPERIMENTAL DESENSITIVE GEL WITH CANNABIDIOL EXTRACT ON THE PROPERTIES AND ULTRAMORPHOLOGY OF BOVINE ENAMEL SURFACE AFTER DENTAL BLEACHING"
-
Data: 17/05/2023
-
Mostrar Resumo
-
The aim of this in vitro study was to evaluate the effect of applied an experimental cannabidiol (CBD)-based desensitizing gel on the properties, ultramorphology and mineral content of bovine enamel bleached with 35% hydrogen peroxide (HP). Forty samples of bovine enamel were prepared and randomly distributed into 4 groups (n=10): G1: Control, G2: HP, G3: KF+HP (2% KF Desensitizer - FGM); G4: CBD+HP. The bleaching agent was applied according to the manufacturer's instructions. The untreated bacterial control group was stored in artificial saliva. Knoop microhardness (KHN), surface roughness (Ra) and color change (∆E) were evaluated using a microdurometer, rugosimeter and spectrophotometer. Enamel morphology and chemical composition were observed under scanning electron microscopy (SEM) and energy dispersive spectrometry (EDS), respectively. Data were tabulated and analysis of variance ANOVA one way and Tukey allowed (p<0.05). All treated groups promoted a significant whitening effect and there was no difference between them for any parameter of color evaluation and surface roughness, but all significantly reduced KHN. It is possible to conclude that the experimental desensitizing CBD gel proved to be a safe option prior to the bleaching agent, as it did not influence the bleaching efficacy, surface roughness, microhardness and mineral content of bovine dental enamel.
Keywords: Cannabidiol ; Dentin Sensitivity ; Dental Enamel; Surface Properties ; Tooth Bleaching.
|
|
|
-
WALLACY WATSON PEREIRA MELO
-
"Mapeamento do conhecimento sobre fotobiomodulação em casos de mucosite oral e avaliação dos efeitos bioquímicos e morfológicos da fotobiomodulação e do açaí em glândulas salivares expostas ao 5-fluoracil".
-
Data: 06/04/2023
-
Mostrar Resumo
-
5-fluorouracil (5-FU) is a chemotherapeutic commonly used in the treatment of different types of solid tumors. It causes numerous adverse effects, both systemic and local. Specifically in the oral cavity, it is associated with mucositis (OM) and salivary gland dysfunctions, which directly impact the patient's quality of life, even influencing the continuity of antineoplastic treatment. In recent years, with the aim of minimizing the deleterious effects of 5-FU, some therapeutic methods have been investigated. One of these is photobiomodulation (FBM), a technique recommended for its good results in stimulating cell proliferation, tissue repair, modulation of the inflammatory process and local analgesia. Another investigated method is the use of natural products, among which is Euterpe oleracea Mart. (EO), known as açaí, rich in bioactive phenolic compounds with antioxidant characteristics. Thus, the present study was divided into two stages. In the first, the objective was to map the 50 most cited articles on the association of FBM and OM. To this end, a search was carried out in the Web of Science Core Collection database, extracting information such as title, authors, average number of citations, study design, year of publication, countries, type of laser used, anatomical points irradiated, parameters of the laser and anticancer therapy. Among studies, clinical trials and literature reviews were the most common types. The main type of laser used was the InGaAlP diode, with a wavelength ranging from 630 to 660nm, power between 40–100 mW and energy density of 0.375–22 J/cm2. The main irradiated anatomical sites were the buccal mucosa, lips, side of the tongue and back of the mouth. In the second stage, an experimental study was carried out, whose objective was to investigate the biochemical and morphological effects of FBM and clarified açaí, used isolated or associated, on the submandibular salivary glands, in a model of OM induced by 5- FU. 102 male Wistar rats were used, randomly divided into 5 experimental groups: Negative Control (CN; n = 6): animals kept under usual laboratory conditions, without intervention; Positive Control (PC; n = 24): animals submitted to chemical induction of OM with 5-FU, but without treatment and that received only gavage with distilled water; FBM (n = 24): animals submitted to OM induction with 5-FU and treated with FBM at a dosage of 6J/cm²; Açaí (n = 24): animals submitted to OM induction with 5- FU and supplemented with açaí clarified by gavage; FBM + Açaí (n = 24): animals submitted to OM induction with 5-FU and that received both FBM (6J/cm²) and açaí clarified by gavage. On day 0, the animals in the CN group were euthanized and submitted to bilateral biopsies of the parotid and submandibular glands, while the animals in the other groups received daily treatments from day 0 to 14. On days 8, 10 and 14, animals from the different groups were They were randomly euthanized and bilateral biopsies of the parotid and submandibular glands were performed for oxidative biochemical analysis (metabolites of nitric oxide - NOx, Antioxidant capacity test against peroxyl radicals - ACAP and Lipid Peroxidation Assay - LPO), morphometric, periodic acid Schiff and histopathological analysis. Data were analyzed using GraphPad Prism v. 8.0, two-way ANOVA and Tukey's post-test, assuming a p-value <0.05. It was shown that, in the submandibular glands, the association of FBM + açaí resulted in an increase in ACAP levels, a reduction in LPO and a decrease in the levels of NOx metabolites, while açaí alone decreased the levels of NOx metabolites. In addition, the association between FBM + açaí was able to maintain the structure of the total area of parenchyma, stroma and acini, maintain the production of mucopolysaccharides and prevent the inflammatory process in the stroma. After the two stages performed, it was possible to conclude, through bibliometric analysis, that in recent years, there has been a growing interest in FBM as a supportive treatment for OM, which provided the evolution of the technique and protocols used. In turn, the experimental study showed that the association of FBM with clarified açaí promoted protection against structural, oxidative biochemical and morphometric damage of the salivary glands exposed to 5-FU. Keywords: oral mucositis; 5-FU; salivary glands; photobiomodulation; clarified açaí.
|
|
|
-
BIANCA NICOLI LOPES DE VASCONCELOS
-
"Influência do polimento dental pós clareamento de consultório no manchamento e rugosidade superficial do esmalte"
-
Data: 03/04/2023
-
Mostrar Resumo
-
The presente study evaluated the influence of dental polishing after in-office bleaching on staining and surface roughness of enamel. Sixty healthy bovine incisors were used and divided into 5 experimental groups, being: G1 – control group (dental bleaching – DB – only); G2 – DB + 2 days coffee staining (STAIN); G3 - DB + 15 days STAIN; G4 – DB + polishing (POL) + 2 days STAIN; G5 - DB + POL + 15 days STAIN. Bleaching was performed using a 35% hydrogen peroxide gel, applied 3 times (15 minutes each) on each bleaching session, in a total of 3 sessions with 7 days time interval between them. Dental polishing was performed using a diamond paste and a soft felt disc under low-speed. Staining was performed immersing dental specimens on a mixture prepared with 15g coffee powder and 250ml boiling distilled water. Enamel color and surface roughness were measured at T0 (baseline), T1 (after dental bleaching) and T2 (after coffee staining). Data were obtained and analyzed using 2-way ANOVA and Tukey’s post hoc test (α=5%). Considering roughness, statistical difference was achieved in all groups when comparing T0 and T2, while a significant one was still observed between T0 and T1 in G4. Colorimetric results exhibited significant differences from T1 to T2 in all groups (except G2) when compared to G1. Therefore, it was possible to conclude that dental polishing was not able to reduce enamel surface roughness, and prevent staining, after in-office bleaching.
Key words: Dental bleaching, Hydrogen peroxide, Dental prophylaxis, Staining.
|
|
|
-
IVANIRO RODRIGUES DA COSTA NETO
-
"ANALYSIS OF THE MORPHOMETRY OF ASTROCYTES OF THE DENTATE GYRUS IN YOUNG MICE SUBMITTED TO ALTERATION OF MASTICATORY ACTIVITY"
-
Data: 03/04/2023
-
Mostrar Resumo
-
In order to investigate the influence of masticatory activity on the morphometry of astrocytes in the middle and inner thirds of the molecular layer of the dentate gyrus (DG), given its relevance as an important input route to the hippocampus, the present study established groups of mice 6-month-old Swiss albinos on three different chewing regimes with variations between hard pelleted diet (HD Hard Diet) and mash diet (SD, Soft Diet): control group (HD), fully on HD diet; group with restricted masticatory function, with half of the temporal window on HD diet and the other half on SD diet (HD/SD); and finally, the group of animals rehabilitated in their masticatory activity, with the initial third of the temporal window on HD diet, changing to SD diet in the second third and returning to HD diet in the third (HD/SD/HD). In each experimental group, 5 animals were analyzed, generating a total, systematic and randomized sample of 447 astrocytes from the area of interest, immunostained with glial fibrillar acidic protein (GFAP) and three-dimensionally reconstructed under optical microscopy. With the morphometric characteristics of the astrocytes, using the hierarchical cluster analysis, we identified two morphological phenotypes in the HD and HD/SD/HD groups and in the HD/SD three phenotypes, designated AST1, AST2 and AST3, which were differentially affected by the studied variables. Which indicates that they perform different functions. The reduction of masticatory activity increased the complexity of astrocytic trees, while the rehabilitation of mastication reversed the increase in complexity to levels lower than that of the control group. All subtypes of the same group showed significant differences in complexity between them (p<0.001), and in the comparison between the different groups, the AST1 subtypes of the HD and HD/SD groups were the only ones that did not reveal a significant difference between their complexities (p=0.113), indicating that subtypes of the same group may be differentially affected by masticatory disorders. We conclude that the alteration of the masticatory activity promotes significant morphological changes in the astrocytes of adult mice, especially in the complexity of the astrocytic trees, which increases in face of the masticatory deficit, presenting even one more cellular subtype in this condition in relation to the other masticatory regimes, we conclude also, that chewing rehabilitation potentially reduces complexity, reversing both this change and the increase in the number of phenotypes identified in masticatory deficit.
keywords:mastication; dentate gyrus; astrocytes; morphometry
|
|
|
-
ADAN LUCAS PANTOJA DE SANTANA
-
"EFEITOS DA TERAPIA DE FOTOBIOMODULAÇÃO (FBM) NA SENSIBILIDADE PÓS-OPERATÓRIA EM CAVIDADES PROFUNDAS DE CÁRIE DENTÁRIA: ESTUDO CLÍNICO RANDOMIZADO DUPLO CEGO"
-
Data: 30/03/2023
-
Mostrar Resumo
-
Introduction: Very deep carious lesions continue to be a challenge in clinical dentistry due to the proximity of the pulp tissue, cavity complexity and reports of postoperative sensitivity. Aim: To evaluate the effects of photobiomodulation therapy (PBMT) on deep cavities of dental caries in patients submitted to minimally invasive treatment (MIT) by selective removal of carious tissue. Methodology: 33 teeth have been evaluated and randomized into three groups. G1: MIT selective caries removal and laser application simulation (placebo); G2: MIT selective caries removal associated with infrared (808nm) low-level laser
therapy; and G3: MIT selective caries removal associated with red (660nm) low- level laser therapy. PBMT effects were assessed by immediate unstimulated
pain sensitivity at 12 hours, 7 days and 14 days. And pain sensitivity stimulated by cold and vertical and horizontal percussion in 14 days, has been assessed by visual analogue scale (VAS). Pain was evaluated using the Kruskal Wallis test with post-test by the Dwass-Steel-Critchlow-Fligner test (intergroup comparison) and the Friedman test (intragroup comparison/non-stimulated pain) and the Wilcoxon test (intragroup comparison/stimulated pain) (p ≤0.05). Results: MIT infrared low-level laser therapy was superior in controlling postoperative pain sensitivity at 7 and 14 days (p=0.042 and p=0.020, respectively). Pain peaks were observed in the MIT group (placebo) at 12 hours and 7 days. At 14 days, stimulated pain was absent in the MIT with and without laser groups. Conclusion: MIT selective caries removal reduces postoperative sensitivity and, when associated with infrared laser photobiomodulation therapy (PBMT), the reduction of postoperative sensitivity in deep cavities of dental caries is superior. Keywords: photobiomodulation therapy; post-surgical sensitivity; post-surgical pain; restorative dentistry; laser therapy.
|
|
|
-
RAFAELA DE ALBUQUERQUE DIAS
-
"EXPRESSION OF STEM CELL MARKERS SALL4, LIN28A AND KLF4 IN AMELOBLASTOMA"
-
Data: 27/03/2023
-
Mostrar Resumo
-
Introduction: Ameloblastoma (AME) is a benign odontogenic tumor of epithelial origin characterized by slow but aggressive growth, infiltrative and recurrent, with the ability to reach large dimensions and invade adjacent structures. In this context, the study of stem cells has shown to be of great importance in the sphere of tumor biology through the possible participation of these cells in progression, invasiveness, angiogenesis and tumor growth. Methodology: Immunohistochemistry was performed on samples of AME, dentigerous cyst (DC) and dental follicle (DF), and indirect immunofluorescence on AME-hTERT cell line to identify the expression of SALL4, LIN28A and KLF4. Results: There was a significant expression of proteins related to cellular pluripotency in AME compared to DC and DF. The analysis revealed that the proteins in question showed high expression, mainly in the parenchyma of tissue samples from the AME and were detected in the AME-hTERT lineage. Conclusion: The results suggest a possible participation of stem cells in the origin, progression and recurrence of AME.
Keywords: ameloblastoma; neoplastic invasiveness; stem cells; immunohistochemistry; immunofluorescence.
|
|
|
-
JONAS IKIKAME DE OLIVEIRA
-
"BREECH GUN TRAUMA TO THE FACE: A RETROSPECTIVE STUDY AND LITERATURE REVIEW"
-
Data: 20/03/2023
-
Mostrar Resumo
-
Facial gunshot wounds due to handmade gun shooting failures are rarely described in the literature and few well-described case series are reported. This paper aims to discuss the consequences of the etiology concerning complications like ocular and neurological damage. This is a retrospective case series of 16 patients attended at a reference trauma center in northern Brazil from 2015 to 2022. A literature review was performed to sediment knowledge about this theme. No statistical analysis revealed a result of p less than 0.05. The victims are exclusively male, have a high rate of facial fractures (93%) and ocular damage (80%), as well as neurological damage and even death as consequence of the etiology. To the best of our knowledge this is the largest report so far. Further studies should be carried out in this etiology, as well as a standardization in the nomenclature to better discuss the subject. Keywords: Gunshot wounds, Maxillofacial Injuries, eye injuries, epidemiology
|
|
|
-
JULIANA COSTA PEREIRA BAIA
-
-
Data: 27/02/2023
-
Mostrar Resumo
-
This study aimed to evaluate the influence of prolonged dental bleaching with 7.5% hydrogen peroxide gel containing calcium (PH) (FGM, Joinville, SC, Brazil) on surface topography, chemical composition, hardness and ultramorphology of dental enamel. A bovine incisor teeth were required, which were divided into the following treatment groups: G1- artificial saliva, G2- dental bleaching (PH), G3 - erosive challenge, G4- abrasive challenge, G5- erosive challenge + abrasive challenge, G6- dental bleaching (PH) + erosive challenge, G7- dental bleaching (PH) + abrasive challenge, G8- dental bleaching (PH) + erosive challenge + abrasive challenge. The analyses of surface loss by profilometry, chemical composition analysis, ultramorphology and microhardness were performed. All groups underwent 3 reading times, being T0- before the beginning of treatments, T1- at the end of the 14th day of treatment (bleaching time recommended by the manufacturer), and T2- at the end of the 28th day of treatment (twice the recommended time). After confirming data normality (Shapiro Wilk test), variance analysis (ANOVA) for dependent samples was performed for all groups submitted to the surface microhardness assay. ANOVA for independent samples (Tukey post-hoc) was performed for all groups submitted to profilometry analysis with surface loss. The data obtained in the SEM and EDS analyses were analyzed qualitatively and through the percentile, respectively. For all analyses undertaken, the level of α significance of 5% was adopted. For the profilometry analysis, an intragroup statistical difference was observed in relation to T0 and T1 between G1, G2 and G5 (p-value ≤0.05) – already in a comparative relationship between T0 and T2, no statistical difference was observed between which group (p-value ≥0.05) - for the evaluation between T1 and T2, it was possible to verify statistical difference between G3, G4, G5, G7 and G8 (p-value ≤0.05). For the microhardness analysis, an intragroup statistical difference was observed between T0, T1 and T2 (p-value ≤0.05) – except in G8 (T1) and G8 (T2) (p-value ≥0.05) and no statistical difference was observed for G1, regardless of the evaluation time (p-value ≥0.05). The SEM analysis showed that in T2, the groups that were exposed to the erosive challenge suffered greater exposure of the prismatic enamel layer, with great irregularity of the peaks and valleys of the prisms – especially in G3. The results obtained from the evaluation of EDS showed lower Ca and P content in G3 and G7, for both evaluation times - being obtained lower absolute value of the chemical elements present in the substrate in G8. Thus, it can be concluded that dental bleaching, although performed for a prolonged period, caused deleterious effects to enamel less pronounced when compared to exposure to acid drink.
Keywords: Dental enamel; Dental bleaching; Optical profilometry and Knoop microhardness.
|
|
|
-
JOÃO DANIEL MENDONÇA DE MOURA
-
"EFEITOS DO AÇAI (EUTERPE OLERACEA MART.) EM ANIMAIS COM LESÃO PERIRRADICULAR INDUZIDA: UM ESTUDO BIBLIOMÉTRICO E EXPERIMENTAL"
-
Data: 23/02/2023
-
Mostrar Resumo
-
The periradicular disease develops as a consequence of pulpal necrosis caused by the entry of microorganisms into radicular canals, resulting from caries, trauma, or iatrogenesis. Oxidative stress plays an important role in the pathogenesis of this disease. Inflammatory mediators are activated by reactive oxygen species, which play a signaling role in the innate immune response. Oxidant imbalance locally contributes to the formation and progression of periapical lesions through direct molecular damage and redox signaling. Based on this, patients affected by periapical lesions are exposed to oxidative stress, which can be extremely dangerous to overall health. This study aimed to evaluate the effect of systemic administration of açaí (Euterpe Oleracea Mart.) on the alveolar bone in healthy animals and with induced periradicular lesions. Initially, a bibliometric study was carried out to synthesize the most frequently cited literature on inducing apical periodontitis in animal models quantitatively and qualitatively. To do this, articles were searched in the Web of Science Core Collection. Only studies on periradicular lesions in animal models were selected, with no language or publication date restrictions. The 50 most cited articles were published between 1965 and 2017 and received between 53 and 1,124 citations in the WoS-CC (a total of 6,189 citations). Stashenko et al. published 14 articles that were cited 1,299 times. Most of the articles originated from North America, while the United States was the most frequent country. "Periapical lesion" was the most frequent keyword. Most of the articles reported using rats, molars, and pulpal exposure to induce periodontitis. The most investigated subject was the mechanism of lesion formation. It was concluded that inducing apical periodontitis in animals has aided in the study of the pathogenesis of apical periodontitis, intracanal medication, systemic repercussion, and regenerative endodontics. Meanwhile, two experimental studies were carried out, one aimed at investigating the effects of açaí gavage in healthy rats, and another aimed at investigating the effects of açaí gavage in rats with induced periapical lesion. For the first study, the animals were divided into a water-gavaged group (Control), an açaí-gavaged group for 14 days (CA14), and a final group gavaged with açaí for 28 days (CA28). Computerized microtomography analyses were performed, evaluating the bone parameters BV, BV/TV, Tb.Th, Tb.N, Tb.Sp, and cortical bone. Histology, where three different staining techniques were performed: hematoxylin/eosin, picrosirius, and Masson's Trichrome. Finally, scanning electron microscopy was performed. Then, statistical analyses were performed using one-way ANOVA followed by Tukey's test. The histological analysis revealed that the CA14 and CA28 groups showed higher osteocyte density and better preservation of collagen fibers than the control group. Furthermore, the açaí-gavaged groups demonstrated higher values of all micro-ct parameters investigated (BV, BV/TV, Tb.th, Tb.N, and Cortical) than the control group, except for Tb.Sp which had a similar value in all groups. For the second experimental study, the animals were divided into six groups, three groups in which the experiment lasted 14 days and three groups in which the experiment lasted 28 days, control (14 and 28 days): healthy animals gavaged with water, LP (14 and 28 days): animals with induced periapical lesion gavaged with water, Açaí + LP (14 and 28 days): animals with induced periapical lesion gavaged with açaí. Blood plasma analyses were performed to analyze results regarding oxidative stress (TBARS, TEAC and GSH); Micro-ct analyses to investigate bone parameters (BV, BV/TV, Tb.Th, Tb.N, Tb.Sp) and lesion volume; Finally, histopathological analyses. Then, statistical analyses were performed by one-way ANOVA followed by Tukey's test. The results showed that the açaí has a systemic antioxidant effect, presenting higher levels of GSH in the two different times studied, lower levels of TBARS only at 28 days, and no difference regarding the TEAC evaluation in both experimental times. Furthermore, the micro-CT parameters showed that the açaí interfered in the lesion volume and in the bone quality parameters in both experimental times, where the animals gavaged with açaí showed smaller lesion volume and better bone parameters than the animals gavaged with water.
Keywords: Açai supplementation; Apical periodontitis; Alveolar bone health; Bibliometric analysis; Oxidative damage.
|
|
|
-
MARILIA DA CUNHA FEIO
-
"O EFEITO DA ATIVIDADE MASTIGATÓRIA, DO ENVELHECIMENTO E DO ENRIQUECIMENTO AMBIENTAL SOBRE A POPULAÇÃO ASTROCITÁRIA DE CA1 E CA3 DO HIPOCAMPO DE CAMUNDONGOS"
-
Data: 15/02/2023
-
Mostrar Resumo
-
The impact of aging, environmental stimulation and a reduction and/or rehabilitation of masticatory activity (HD/SD and HD/SD/HD) on spatial memory and learning has been studied, but little is known about the influence of these variables on astrocytes from different regions and hippocampal subcamps. The present study therefore aims to identify the estimate of the amount of astrocytes in stratum radiatum, stratum lacunosum-moleculare and stratum oriens of CA1 and CA3 of the hippocampus on the influence of age, environment (enriched or depleted), and chewing (presence and absence) in mus muscle mice. The experimental group consisted of 60 female albino Swiss mice that were distributed into age groups of 6 months and 18 months, being housed in impoverished (standard environment) and enriched (enriched environment) conditions and received, after weaning, to 3 types of different diets: pelleted feed (HD), feed pelleted followed by mash (HD/SD) and pelleted/mashed/pelleted feed (HD/SD/HD). When completing the temporal window age, the mice were sacrificed for immunohistochemical processing for glial fibrillar acidic protein (GFAP), used for the identification of astrocytes. The estimate of the amount of astrocytes was done through design-based stereology using the optical fractionator, the results obtained were admitted to statistical analysis using the 3-way Anova test and Tukey test considering a 5% alpha with p<0.05. Results observed that aging was able to significantly reduce the amount of astrocytes in the stratum radiatum of CA1 for mice reared in a standard environment (IE) with HD diet and that masticatory changes (HD/SD and HD/SD/HD) increased the amount of astrocytes in stratum radiatum from CA1 for elderly animals (18M) reared in standard environment (IE). There were no changes in CA3. Key-words: astrocyte, mastication, hippocampus, diet,
|
|
|
-
KAROLYNY MARTINS BALBINOT
-
TGF-ΒRII/SMAD4 SIGNALING PATHWAY IS RELATED TO CELLULAR PLURIPOTENCE AND THE BIOLOGICAL BEHAVIOR OF AMELOBASTOMA
-
Data: 14/02/2023
-
Mostrar Resumo
-
Introduction: Ameloblastoma (AME) is a locally aggressive odontogenic neoplasm. Identifying the characteristics of self-renewal, pluripotency, as well as the pathway responsible for promoting these stimuli in EBF, is relevant. Methodology: To identify the expression of SOX2, NANOG, OCT4, TGFβ1, TGFβRII and SMAD-4 in SMA, dentigerous cyst (DC) and dental follicle (DF) samples, immunohistochemistry was performed. TGFβRII silencing was obtained in the AME-hTERT strain, followed by indirect immunofluorescence, cell viability and proliferation assay, and wound assay. Results: There was significant expression in AME of proteins related to cellular pluripotency and those that make up the TGFβRII/SMAD-4 pathway. The high expression of the studied proteins was located predominantly in the parenchyma of the AME tissue samples, as well as expressed in the AME-hTERT lineage. The silencing of TGFβRII had repercussions both on the expression of proteins in the pathway and on the expression of proteins directly related to pluripotency, causing a decrease in cell proliferation and migration. Conclusion: It was possible to demonstrate the pluripotent potential present in AME cells and provide evidence that the TGFβR2/SMAD-4 signaling pathway is involved in the regulation of factors related to cellular pluripotency in AME, favoring its progression and possibly increasing its rates of recurrence.
Key words: Ameloblastoma, TGF-β/Smad signaling, Cellular pluripotency, Biological behavior.
|
|
|
-
ROBERTO CARLOS RIVADANEIRA CÁRDENAS
-
Three-dimensional evaluation of condylar anatomical changes during mandibular setback and advancement after the Surgery First Orthognatic Protocol
-
Data: 30/01/2023
-
Mostrar Resumo
-
Introduction: Condylar resorption after orthognathic surgery can be defined as a progressive change in the anatomy of the mandibular condyles, in shape and volume of the mandibular condyle. It is a multifactorial process that may be associated with surgical or non-surgical factors. These effects have a higher incidence in cases of Double Jaw surgeries. It may cause loss of condylar height and skeletal recurrence, leading to the reestablishment of the pre-surgical condition or an unfavorable evolution. Methodology: The method will describe volumetric condylar changes comparing mandibular setback and advancement after orthognathic surgeries in 30 patients that are part of the Ophir Loyola Hospital database. It will compare the superimposition of digitized images obtained from computed tomography in two different time periods, which are: the preoperative period and the 180-day postoperative period. Softwares were used for aligning and segmenting the condyles, for virtual planning, for superimposition and for calculating image discrepancies using reference points. The statistical test chosen was Student's t test for individual samples. Group A includes patients operated by the Surgery First Orthognatic Protocol. Group B, represented by patients operated by the Conventional Orthognatic Surgery Technique. Results: Concerning the Surgery First Orthognatic Protocol, we obtained an average of 0.3957 in the right condyle and an average of 0.3861 in the left condyle. Concerning the Conventional Orthognatic Surgery Technique, we obtained an average of 0.3189 on the right condyle and an average of 0.3831 on the left condyle. Statistically, there was no difference between the two groups (p = 0.27). Conclusion: No significant changes were observed in the anatomy of the condyles comparing the Surgery First Orthognatic Protocol and the Conventional Orthognatic Surgery Technique.
Keywords: Dentofacial Anomalies, Orthognatic Surgery, Mandibular Condyle, Softwares, Diagnostic Imaging.
|
|